Given the situation and the available resources, PCR would be the better choice for investigating the outbreak of diarrheal food poisoning caused by a bacterial infection.
API-20E is a commonly used biochemical test kit that identifies various strains of Enterobacteriaceae, including Salmonella, Shigella, and pathogenic strains of E. coli, among others. However, it has some limitations, such as being time-consuming and requiring a significant amount of bacterial growth for testing.
PCR (polymerase chain reaction), on the other hand, is a molecular technique that can rapidly detect and amplify DNA sequences specific to a particular pathogen, allowing for quick and sensitive identification of the infectious agent responsible for the outbreak. PCR can detect a single copy of the target DNA, making it highly sensitive and specific. Moreover, PCR does not require bacterial growth, which means that even small amounts of the infectious agent in the 75%-alcohol-treated leftovers of food eaten by the outbreak victims can be detected.
Therefore, given the time and sensitivity requirements of this investigation, PCR would be the better choice over API-20E for identifying the infectious agent responsible for the outbreak of diarrheal food poisoning in the local restaurant. |